RNA-Targeted Therapies and High-Throughput Screening Methods

Methods for High-Content, High-Throughput Image-Based Cell Screening

Visual inspection of cells is a fundamental tool for discovery in biological science. Modern robotic microscopes are able to capture thousands of images from massively parallel experiments such as RNA interference (RNAi) or small-molecule screens. Such screens also benefit from lab automation, making large screens, e.g., genome-scale knockdown experiments, more feasible and common. As such, the...

RNA-Targeted Therapies and Amyotrophic Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS) is a fatal motor disease in adults. Its pathophysiology remains mysterious, but tremendous advances have been made with the discovery of the most frequent mutations of its more common familial form linked to the C9ORF72 gene. Although most cases are still considered sporadic, these genetic mutations have revealed the role of RNA production, processing and tra...

High-throughput and parallel screening methods in asymmetric hydrogenation.

High-throughput and parallel screening methods are being used more and more extensively in various areas of chemistry, including enantioselective synthesis.1 This due to the extremely small energy differences determining such critical factors as enantioselectivity. Yue and Nugent2 (Scheme 1) and Boulton et al.3 (Scheme 2) recently described two examples, which illustrate this quite well. In bot...

High throughput screening. Methods and protocols, second edition. Preface.

High Throughput Screening and Selection Methods for Directed Enzyme Evolution

Successful evolutionary enzyme engineering requires a high throughput screening or selection method, which considerably increases the chance of obtaining desired properties and reduces the time and cost. In this review, a series of high throughput screening and selection methods are illustrated with significant and recent examples. These high throughput strategies are also discussed with an emp...